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recombinant mouse igg1 isotype control antibody  (InvivoGen)


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    Structured Review

    InvivoGen recombinant mouse igg1 isotype control antibody
    ( A ) Quantified levels of IL-1α from naïve fibroblast (Naïve fibro), inflammatory fibroblast (Inflam fibro), and ectopic basal cell (Basal) conditioned media as determined by ELISA. ( B ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates and treated with a 5-fold titration of IL-1α added to control media, including the level determined in (A). ( C ) Graphic illustrating the strategy used to deplete conditioned media of endogenous IL-1α with antibodies, magnetic beads, and column-based separation. ( D ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates in control or conditioned media depleted using <t>IgG</t> (CTRL IgG or CM IgG) or IL-1α nAb (CTRL nAB, CM nAb) and supplemented with additional IL-1α nAb after depletion (+Spike). [(B) and (D)] Data shows mean fold change ± SD and are pooled from 3 independent experiments (n=3 mice). P values were calculated using either (B) a ratio paired t -test or (D) a one-way ANOVA with post-hoc Turkey multiple comparison test: * P < 0.05, ** P < 0.01, **** P < 0.0001, ns: not significant.
    Recombinant Mouse Igg1 Isotype Control Antibody, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+migg1/Anti-%CE%B2-Gal-mIgG1/bio_rxiv__64898__2026__04__02__716135-221-39-45
    Average 94 stars, based on 4 article reviews
    recombinant mouse igg1 isotype control antibody - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Dysplastic Epithelial Repair Propagates Chronic Pathology Through the Paracrine Transformation of Pulmonary Fibroblasts"

    Article Title: Dysplastic Epithelial Repair Propagates Chronic Pathology Through the Paracrine Transformation of Pulmonary Fibroblasts

    Journal: bioRxiv

    doi: 10.64898/2026.04.02.716135

    ( A ) Quantified levels of IL-1α from naïve fibroblast (Naïve fibro), inflammatory fibroblast (Inflam fibro), and ectopic basal cell (Basal) conditioned media as determined by ELISA. ( B ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates and treated with a 5-fold titration of IL-1α added to control media, including the level determined in (A). ( C ) Graphic illustrating the strategy used to deplete conditioned media of endogenous IL-1α with antibodies, magnetic beads, and column-based separation. ( D ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates in control or conditioned media depleted using IgG (CTRL IgG or CM IgG) or IL-1α nAb (CTRL nAB, CM nAb) and supplemented with additional IL-1α nAb after depletion (+Spike). [(B) and (D)] Data shows mean fold change ± SD and are pooled from 3 independent experiments (n=3 mice). P values were calculated using either (B) a ratio paired t -test or (D) a one-way ANOVA with post-hoc Turkey multiple comparison test: * P < 0.05, ** P < 0.01, **** P < 0.0001, ns: not significant.
    Figure Legend Snippet: ( A ) Quantified levels of IL-1α from naïve fibroblast (Naïve fibro), inflammatory fibroblast (Inflam fibro), and ectopic basal cell (Basal) conditioned media as determined by ELISA. ( B ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates and treated with a 5-fold titration of IL-1α added to control media, including the level determined in (A). ( C ) Graphic illustrating the strategy used to deplete conditioned media of endogenous IL-1α with antibodies, magnetic beads, and column-based separation. ( D ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates in control or conditioned media depleted using IgG (CTRL IgG or CM IgG) or IL-1α nAb (CTRL nAB, CM nAb) and supplemented with additional IL-1α nAb after depletion (+Spike). [(B) and (D)] Data shows mean fold change ± SD and are pooled from 3 independent experiments (n=3 mice). P values were calculated using either (B) a ratio paired t -test or (D) a one-way ANOVA with post-hoc Turkey multiple comparison test: * P < 0.05, ** P < 0.01, **** P < 0.0001, ns: not significant.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Gene Expression, Quantitative RT-PCR, Cell Culture, Titration, Control, Magnetic Beads, Comparison

    Related Articles

    Control:

    Article Title: A Proinflammatory Function of Toll-Like Receptor 2 in the Retinal Pigment Epithelium as a Novel Target for Reducing Choroidal Neovascularization in Age-Related Macular Degeneration
    Article Snippet: Cells at passages 1 to 3 were used for experiments; they were often pigmented and displayed a transepithelial electrical resistance of at least 500 Ω·cm 2 (EVOM2 Voltohmmeter; World Precision Instruments, Inc., Sarasota, FL) as described. .. 43 The synthetic TLR2 ligand PAM2CSK4, neutralizing anti-TLR2 antibody (MAb-mTLR2), anti-TLR1 antibody (Anti–hTLR1-IgG), anti-TLR6 antibody (Anti–hTLR6-IgG), and control mIgG1 were obtained from InvivoGen. .. The high purity (≥98% based on nuclear magnetic resonance analysis) CEP-dipeptide was obtained from a commercial source as a custom synthesis (Haoyuan Chemexpress Co., Limited, Shanghai, P.R.

    Article Title: A Proinflammatory Function of Toll-Like Receptor 2 in the Retinal Pigment Epithelium as a Novel Target for Reducing Choroidal Neovascularization in Age-Related Macular Degeneration.
    Article Snippet: Once cells reached confluence (1 to 2 weeks; passage 0), they were seeded into Transwell plates (12-mm insert, 400-nm pores; Corning Inc., Corning, NY) that were coated with laminin extracellular matrix (Sigma-Aldrich) at 1.5 to 2.0 105 cells/well. .. Cells at passages 1 to 3 were used for experiments; they were often pigmented and displayed a transepithelial electrical resistance of at least 500 U$cm2 (EVOM2 Voltohmmeter; World Precision Instruments, Inc., Sarasota, FL) as described.43 The synthetic TLR2 ligand PAM2CSK4, neutralizing anti-TLR2 antibody (MAb-mTLR2), anti-TLR1 antibody (AntiehTLR1-IgG), anti-TLR6 antibody (AntiehTLR6IgG), and control mIgG1 were obtained from InvivoGen. .. The high purity ( 98% based on nuclear magnetic resonance analysis) CEP-dipeptide was obtained from a commercial source as a custom synthesis (Haoyuan 2212 Chemexpress Co., Limited, Shanghai, P.R.



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    ( A ) Quantified levels of IL-1α from naïve fibroblast (Naïve fibro), inflammatory fibroblast (Inflam fibro), and ectopic basal cell (Basal) conditioned media as determined by ELISA. ( B ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates and treated with a 5-fold titration of IL-1α added to control media, including the level determined in (A). ( C ) Graphic illustrating the strategy used to deplete conditioned media of endogenous IL-1α with antibodies, magnetic beads, and column-based separation. ( D ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates in control or conditioned media depleted using <t>IgG</t> (CTRL IgG or CM IgG) or IL-1α nAb (CTRL nAB, CM nAb) and supplemented with additional IL-1α nAb after depletion (+Spike). [(B) and (D)] Data shows mean fold change ± SD and are pooled from 3 independent experiments (n=3 mice). P values were calculated using either (B) a ratio paired t -test or (D) a one-way ANOVA with post-hoc Turkey multiple comparison test: * P < 0.05, ** P < 0.01, **** P < 0.0001, ns: not significant.
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    HEK TLR2 ( a ) and VK2 vaginal epithelial cells ( b ) were incubated with bacteria (MOI = 10) overnight. TLR1 and TLR6-dependence was investigated by pre-incubating the cells for 30 min with 1 μg/ml anti-TLR1, anti-TLR6 or <t>mIgG1</t> isotype control antibodies before overnight stimulation with the bacteria at MOI = 10. Fv Fannyhessea vaginae , Fn Fusobacterium nucleatum , Pb Prevotella bivia , Sv Sneathia vaginalis , Mm Mobiluncum mulieris , Fm Fingoldia magna , Va Veillonella atypica . Data are mean +/−SD ( n = 3 biological replicates). Two-way ANOVA, Tuckey multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0001. Source data are provided as a Source Data file.
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    Image Search Results


    ( A ) Quantified levels of IL-1α from naïve fibroblast (Naïve fibro), inflammatory fibroblast (Inflam fibro), and ectopic basal cell (Basal) conditioned media as determined by ELISA. ( B ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates and treated with a 5-fold titration of IL-1α added to control media, including the level determined in (A). ( C ) Graphic illustrating the strategy used to deplete conditioned media of endogenous IL-1α with antibodies, magnetic beads, and column-based separation. ( D ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates in control or conditioned media depleted using IgG (CTRL IgG or CM IgG) or IL-1α nAb (CTRL nAB, CM nAb) and supplemented with additional IL-1α nAb after depletion (+Spike). [(B) and (D)] Data shows mean fold change ± SD and are pooled from 3 independent experiments (n=3 mice). P values were calculated using either (B) a ratio paired t -test or (D) a one-way ANOVA with post-hoc Turkey multiple comparison test: * P < 0.05, ** P < 0.01, **** P < 0.0001, ns: not significant.

    Journal: bioRxiv

    Article Title: Dysplastic Epithelial Repair Propagates Chronic Pathology Through the Paracrine Transformation of Pulmonary Fibroblasts

    doi: 10.64898/2026.04.02.716135

    Figure Lengend Snippet: ( A ) Quantified levels of IL-1α from naïve fibroblast (Naïve fibro), inflammatory fibroblast (Inflam fibro), and ectopic basal cell (Basal) conditioned media as determined by ELISA. ( B ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates and treated with a 5-fold titration of IL-1α added to control media, including the level determined in (A). ( C ) Graphic illustrating the strategy used to deplete conditioned media of endogenous IL-1α with antibodies, magnetic beads, and column-based separation. ( D ) Saa3 and Ccl2 gene expression by RT-qPCR for fibroblasts cultured on 0.5kPa plates in control or conditioned media depleted using IgG (CTRL IgG or CM IgG) or IL-1α nAb (CTRL nAB, CM nAb) and supplemented with additional IL-1α nAb after depletion (+Spike). [(B) and (D)] Data shows mean fold change ± SD and are pooled from 3 independent experiments (n=3 mice). P values were calculated using either (B) a ratio paired t -test or (D) a one-way ANOVA with post-hoc Turkey multiple comparison test: * P < 0.05, ** P < 0.01, **** P < 0.0001, ns: not significant.

    Article Snippet: The working concentration for the following reagents can be found in the text and figure legends: Human IL-1RA Recombinant Protein (IL1Ra) (PeproTech®, 200-01RA), Mouse IL-1 alpha Recombinant Protein, (PeproTech®, 211-11A), Mouse IL-1α Neutralizing Antibody (Invivogen, Anti-mIL-1α-mIgG1 clone 6H7, mIL-1α-mab9-02), Recombinant Mouse IgG1 Isotype Control Antibody (Invivogen, Anti-β-Gal-mIgG1 clone T9C6, bgal-mab9-02), Human TGF-β 1 Recombinant Protein (PeproTech®, 100-21), Human GDF15 Recombinant Protein (PeproTech®, 120-28C), Human VEGF Recombinant Protein (PeproTech®, 450-32), Human OPN Recombinant Protein (PeproTech®, 120-35), Murine CXCL1 Recombinant Protein (PeproTech®, 250-11), Human IGFBP3 Recombinant Protein (PeproTech®, 100-08), Human TNFRSF11B Recombinant Protein (PeproTech®, 450-14), Murine CXCL5 Recombinant Protein (PeproTech®, 250-17), and Muring CCL20 Recombinant Protein (PeproTech®, 250-27).

    Techniques: Enzyme-linked Immunosorbent Assay, Gene Expression, Quantitative RT-PCR, Cell Culture, Titration, Control, Magnetic Beads, Comparison

    HEK TLR2 ( a ) and VK2 vaginal epithelial cells ( b ) were incubated with bacteria (MOI = 10) overnight. TLR1 and TLR6-dependence was investigated by pre-incubating the cells for 30 min with 1 μg/ml anti-TLR1, anti-TLR6 or mIgG1 isotype control antibodies before overnight stimulation with the bacteria at MOI = 10. Fv Fannyhessea vaginae , Fn Fusobacterium nucleatum , Pb Prevotella bivia , Sv Sneathia vaginalis , Mm Mobiluncum mulieris , Fm Fingoldia magna , Va Veillonella atypica . Data are mean +/−SD ( n = 3 biological replicates). Two-way ANOVA, Tuckey multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0001. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Lactobacillus crispatus S-layer proteins modulate innate immune response and inflammation in the lower female reproductive tract

    doi: 10.1038/s41467-024-55233-7

    Figure Lengend Snippet: HEK TLR2 ( a ) and VK2 vaginal epithelial cells ( b ) were incubated with bacteria (MOI = 10) overnight. TLR1 and TLR6-dependence was investigated by pre-incubating the cells for 30 min with 1 μg/ml anti-TLR1, anti-TLR6 or mIgG1 isotype control antibodies before overnight stimulation with the bacteria at MOI = 10. Fv Fannyhessea vaginae , Fn Fusobacterium nucleatum , Pb Prevotella bivia , Sv Sneathia vaginalis , Mm Mobiluncum mulieris , Fm Fingoldia magna , Va Veillonella atypica . Data are mean +/−SD ( n = 3 biological replicates). Two-way ANOVA, Tuckey multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0001. Source data are provided as a Source Data file.

    Article Snippet: To investigate TLR1, TLR2 and TLR6 dependence, VK2/E6E7 cells were pre-incubated for 30 min at 37 °C with 1 μg/ml of anti-hTLR1 (mabg-htlr1, clone H2G2, InvivoGen), anti-hTLR2 (MAB2616, clone # 383936, R&D Systems) and anti-hTLR6 (mabg-htlr6, clone C5C8, InvivoGen) antibody or mIgG1 (InvivoGen, clone T8E5, mabg1-ctrlm) and mIgG2b (14-4732-85, lot 2288614, clone eBMG2b, ThermoFisher Scientific) isotype controls.

    Techniques: Incubation, Bacteria, Control, Comparison

    a SDS-PAGE gel of crude SLPs extracted with LiCl 5 M from L. crispatus and L. iners isolates. 10μg total proteins were loaded per lane. Representative of three independent experiments. b HEK-TLR2 cells were stimulated for 16 h with bacteria (MOI = 10) and NF-κB activation was determined by measuring alkaline phosphatase activity and reading O.D. at 630 nm. c VK2 vaginal epithelial cells were stimulated for 16 h and IL-8 release in the supernatant was quantified by ELISA. d HEK TLR2 cells were stimulated with lithium chloride-treated bacteria (MOI = 10) for 16 h and IL-8 release in the supernatant was quantified by ELISA. TLR1, TLR2 and TLR6 dependence was investigated by pre-incubating cells for 30 min at 37 °C with 1 μg/ml of anti-TLR1, anti-TLR2, anti TLR6 or mIgG1 and mIgG2b isotype control antibodies. b – d A representative figure of three independent experiments is shown (mean +/− SD). Two-way ANOVA, Tuckey multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0,001. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Lactobacillus crispatus S-layer proteins modulate innate immune response and inflammation in the lower female reproductive tract

    doi: 10.1038/s41467-024-55233-7

    Figure Lengend Snippet: a SDS-PAGE gel of crude SLPs extracted with LiCl 5 M from L. crispatus and L. iners isolates. 10μg total proteins were loaded per lane. Representative of three independent experiments. b HEK-TLR2 cells were stimulated for 16 h with bacteria (MOI = 10) and NF-κB activation was determined by measuring alkaline phosphatase activity and reading O.D. at 630 nm. c VK2 vaginal epithelial cells were stimulated for 16 h and IL-8 release in the supernatant was quantified by ELISA. d HEK TLR2 cells were stimulated with lithium chloride-treated bacteria (MOI = 10) for 16 h and IL-8 release in the supernatant was quantified by ELISA. TLR1, TLR2 and TLR6 dependence was investigated by pre-incubating cells for 30 min at 37 °C with 1 μg/ml of anti-TLR1, anti-TLR2, anti TLR6 or mIgG1 and mIgG2b isotype control antibodies. b – d A representative figure of three independent experiments is shown (mean +/− SD). Two-way ANOVA, Tuckey multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0,001. Source data are provided as a Source Data file.

    Article Snippet: To investigate TLR1, TLR2 and TLR6 dependence, VK2/E6E7 cells were pre-incubated for 30 min at 37 °C with 1 μg/ml of anti-hTLR1 (mabg-htlr1, clone H2G2, InvivoGen), anti-hTLR2 (MAB2616, clone # 383936, R&D Systems) and anti-hTLR6 (mabg-htlr6, clone C5C8, InvivoGen) antibody or mIgG1 (InvivoGen, clone T8E5, mabg1-ctrlm) and mIgG2b (14-4732-85, lot 2288614, clone eBMG2b, ThermoFisher Scientific) isotype controls.

    Techniques: SDS Page, Bacteria, Activation Assay, Activity Assay, Enzyme-linked Immunosorbent Assay, Control, Comparison